马尾松体细胞胚胎发生相关基因PmSERKl的克隆与表达分析

体细胞胚胎发生不仅是植物微繁、种质保存的有效手段，同时也是研究高等植物胚胎发育和遗传转化的模式系统之一。本研究从马尾松未成熟合子胚诱导的胚性细胞团中成功克隆了体胚发生受体激酶基因PmSERK1。该基因全长2303bp，包含完整的开放阅读框，编码626个氨基酸，其蛋白分子量约为69kD。序列分析发现PmSERK1与其它植物的SERK基因高度同源。系统进化树重建表明，PmSERK1与已知参与体胚发生的关键SERK基因聚为一类，如MtSERK1、CuSERK1、AtSERK，和DcSERK。表达分析显示：PmSERK1在主要的组织器官（根、茎、针叶等）略有表达；但在诱导培养的非胚性愈伤中没有检测到表达，而在经继代培养的胚性愈伤中表达很高且逐渐增加，且在培养20d时达到最高。另外，在再生的子叶胚中，PmSERK1的表达量也很高。综上所述表明，PmSERK1是与体胚发生相关的关键SERK基因的直系同源基因，可以作为愈伤组织胚性的标记基因。     

透光抚育对张广才岭“栽针保阔”红松林群落生产力的影响

介绍了透光抚育对不同立地(不同坡位)上的栽针保阔红松林群落的公顷蓄积和生产力的影响,研究分析如何通过不同强度透光抚育提高栽针保阔红松林群落生产力。     

灌木围障预防兔害对油松高影响研究

产生兔害的根本原因是竞争有限的空间和食物资源,灌木围障将野兔与林木隔离,不限制其在林内的活动,是一种环境友好型的林木兔害预防方法.为了掌握灌木围障对林木高的影响,研究了用沙棘、黄刺玫和其他灌木枝条围罩处理对油松高的作用.试验在当年油松定植时布设,设罩盖与不罩盖处理,5次重复,苗龄3 a,各处理300株.2006年5月定植时对处理区的油松进行测量、标记.处理后2007-2011年5月各调查1次油松高生长量.结果表明:灌木围障隔离对油松高有促长作用,且灌木篱笆促长作用优于灌木围墙.桥北灌木篱笆区定植5 a的油松综合增益指数为20.6％±1.35％,比灌木围墙处理提高了9.3％±1.90％,差异极显著(F=23.299,p=0.000).而旬邑林区两种处理间油松净高增益指数差异不显著(F=2.429,p=0.126),综合增益指数相差2.7％±1.03％.两地各处理油松净高增益大小依次为桥北篱笆＞桥北围墙＞旬邑篱笆＞旬邑围墙.随着定植年限的延长,两地油松净高增益指数有进一步扩大的趋势,油松高生长差距逐渐扩大.     

福建省马尾松优树自由授粉子代变异及其分析

对１０年生马尾松优树自由授粉子代的树高、胸径、材积和通直度性状变异的研究结果表明，２６３个优树家系中有２６．６２％材积生长超过对照１０％以上．其中龙岩地区优树的子代生长表现最佳．同地区不同林分的优树子代有很大差异，林分间平均变异系数大于林分内家系间的平均变异系数．在不同海拔林分选择的优树，除戴云山南麓海拔６００～７００ｍ左右优树子代生长表现较好外，其它海拔点的子代无明显差异，且都差于对照．优树子代生长性状与树干通直度之间存在显著正相关，表明可望同时在生长性状和干形上得到改良     

降水过程对飞播造林油松成苗的影响

为了找出影响飞播油松成苗的主要因素，采用随机抽样调查方法和机械抽样调查方法，对滦平县历年的飞播区进行了调查分析。结果表明，降水过程是影响飞播油松成苗的主要因素。     

Allelopathic Effect of Eucalyptus globulus Labill. on Cyperus rotundus L. and Cynodon dactylon L. Pers.

The allelopathic potential of Eucalyptus globulus Labill. (gum tree) fresh and dried leaf leachates was studied using two perennial weeds, viz. purple nutsedge (Cyperus rotundus L.) and bermuda grass (Cynodon dactylon L. Pers) as test weeds. Aqueous leachate of fresh leaves of eucalyptus significantly suppressed the establishment of vegetative propagules and early seedling growth of the weeds. Leachate of fresh leaf cuttings had growth inhibitory effect on bermuda grass but showed growth promotion effect on purple nutsedge. Similarly the leachate of dried leaves of eucalyptus had differential influence on the growth of the two weeds. There is a possibility to harness the allelochemicals of eucalyptus leaves as herbicides for the management of these perennial weeds.     

松树针叶束内源激素与单宁的变化

本文分析和比较了油松、白皮松、华山松和樟子松各种针叶束内源激素与单宁含量的变化。结果表明，ＡＢＡ与单宁含量在树种间、树龄间和针叶束叶龄间均存在明显差异，针叶束经低温砂藏处理，其内源激素与单宁水平呈规律性变化，在处理前期，和树种针叶束的ＬＡＡ、ＧＡｓ、Ｚ和ＺＲ水平明显提高，然后随砂藏时间的延长而趋于降低；而ＡＢＡ与单宁水平则呈相反的变化，在砂藏过程中内源激素与单宁的变化规律与前期实验中经砂藏处理的离体针叶束不定根的诱导率紧密相关。结果表明，低温砂藏处理在一定时期可以促使松树针叶束内源激素与单宁水平的协调性变化，这种变化对离体针叶束不定根的诱导具有明显的促进作用     

用模糊聚类法分析松毛虫个体变异及种类

应用模糊聚类方法,分析了湖南宁乡、永州,福建南安,广西钦州和四川永川、西昌等地松毛虫种群的生殖器的个体变异和种群类型。结果表明,在同一种群个体间存在着差异,有的种群个体差异显著,有的则不甚显著。各地种群在置信水平λ值为0.616 430 6时,可以聚为一类。     

采脂资源变化与技术革新

本文论述了我国马尾松松脂资源变化的趋势，指出我国南方主产脂区马尾松成、过熟林已所剩无几，本世纪末中龄马尾松人工林将成为采脂的主力军，但采脂技术水平低下，管理落后.针对上述问题提出了适合于中龄林采脂的新技术及集约化中龄林采脂基地管理新体系.     

Bark beetle community structure under four ponderosa pine forest stand conditions in northern Arizona

We studied the bark beetle guild (Coleoptera: Scolytidae) in the ponderosa pine forests of northern Arizona to explore if the species assemblages and relative abundance differ between managed and unmanaged stands. Four stand conditions were assessed: (1) unmanaged stands with high tree density, (2) thinned stands, (3) thinned and burned (with prescribed fire) stands and (4) stands that had been burned by stand replacing wildfires. The study was conducted in the ponderosa pine forests of the Coconino Plateau, northern Arizona. For several decades this area has been relatively free of bark beetle outbreaks despite the current overstocked condition of many stands. We found that a similar species assemblage composed of Dendroctonus frontalis, D. brevicomis, D. valens, D. approximatus, D. ponderosae, and Ips pini occurred across all four stand conditions over 3 years of study. The population levels of all these species were endemic across all stand conditions. The non-aggressive D. approximatus and D. valens were indicator species for thinned and unmanaged stands, respectively, but this was not consistent among years. The ambrosia beetle Gnathotrichus sp. and the bark beetle predator Enoclerus sp. consistently indicated stands burned by wildfire. In addition to our field experiment, we analyzed the historical pattern of attacks of bark beetles in our area of study. Our findings suggest that the pattern of attack of D. brevicomis (the only Dendroctonus species for which attacks have been reported) and Ips spp. has been through scattered small infestations in groups of 1–10 trees. Whereas small infestations by Ips spp. are increasing, those for D. brevicomis are decreasing. Although we agree that the high density stands in northern Arizona are in an “unhealthy” condition, our results do not show that they were supporting large bark beetle outbreaks. Our results challenge the theoretical assumptions about the relationship between stand structure, tree resistance and bark beetle performance.